![]() (2006) Eastern blotting has been used to refer to the detection of fusion proteins through complementation.The method is similar to southwestern blot. This could be seen as similar to a Southern blot, however the interaction is between a DNA molecule (the aptamer) and a protein, rather than two DNA molecules. (2005) Eastern blot has been used to describe a blot of proteins on polyvinylidene fluoride membrane where the probe is an aptamer rather than an antibody.Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2002) Eastern blot has also been used to describe an immunoblot performed on proteins blotted to a polyvinylidene fluoride membrane from a PAGE gel run with opposite polarity.The method is essentially far-eastern blot. This method has also been discussed in later work by the same group. The membrane is then probed with antibodies for epitopes of interest. The oxidized protein is then treated with a complex mixture, generating a new conjugate on the membrane. (2001) Eastern blotting was described as a technique for detecting glycoconjugates generated by blotting BSA onto polyvinylidene fluoride membranes, followed by periodate treatment.The method is described more fully in the article on far-eastern blot, but is based on antibody or lectin staining of lipids transferred to polyvinylidene fluoride membranes. (2000) Far-eastern blotting seems to have been first named in 2000 by Ishikawa & Taki.in 1994, which they originally dubbed TLC blotting, and was based on a similar method introduced by Towbin in 1984. This method is based on earlier work by Taki et al. The method involved blotting of phospholipids on polyvinylidene fluorideor nitrocellulose membrane prior to transfer of proteins onto the same nitrocellulose membrane by conventional western blotting and probing with conformation specific antibodies. (1996) Eastern-western blot was first used by Bogdanov et al.The immobilized RNA is then probed using DNA. (1984) Middle-eastern blotting has been described as a blot of polyA RNA (resolved by agarose) which is then immobilized.(1982) The term eastern blotting was specifically rejected by two separate groups: Reinhart and Malamud referred to a protein blot of a native gel as a native blot Peferoen et al., opted to refer to their method of drawing sodium dodecyl sulfate-gel separated proteins onto nitrocellulose using a vacuum as Vacuum blotting.In some cases, the technique had been in practice for some time before the introduction of the term. The current definitions are summarized below in order of the first use of the name however, all are based on some earlier works. All of the definitions are a derivative of the technique of western blot developed by Towbin in 1979. History and multiple definitions Äefinition of the term eastern blot is somewhat confused due to multiple sets of authors dubbing a new method as eastern blot, or a derivative thereof. ![]() In principle, eastern blotting is similar to lectin blotting (i.e., detection of carbohydrate epitopes on proteins or lipids). Eastern blotting should be used to refer to methods that detect their targets through specific interaction of the post-translational modifications and the probe, distinguishing them from a standard far-western blot. Transferred proteins are analyzed for post-translational modifications using probes that may detect lipids, carbohydrate, phosphorylation or any other protein modification. Multiple techniques have been described by the term "eastern blot(ting)", most use phosphoprotein blotted from sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel on to a polyvinylidene fluoride or nitrocellulose membrane. Thus, eastern blot can be considered an extension of the biochemical technique of western blot. It is most often used to detect carbohydrate epitopes. The eastern blot, or eastern blotting, is a biochemical technique used to analyze protein post-translational modifications including the addition of lipids, phosphates, and glycoconjugates.
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